24 hrs should be enough but if brood rearing in your hives has dropped the queen might not co-operate
When there is a nectar flow and the brood is expanding the queen lays in the cells with no problem
borrowed this link from another forum
http://www.chitwoodfamilyfarm.com/nicot.html
Sent from my LIFETAB_S1034X using Tapatalk
>I just want to ask for the proper timing for confining the queen in the jenter box, is it in the morning or evening?
It doesn't matter. Just remove her 24 hours later and transfer the larvae four days later. If you confined her in the morning then plan on morning to release her if it is laid up. If not, wait another day.
> And when to take the plugs best timing for best results
Four days after you confined her if the eggs have hatched. If not, wait another day for them to hatch.
http://www.bushfarms.com/beesqueenrearing.htm#calendar
Thanks master, I appreciate your advice that's what I needed to know, thanks again
Sent from my D6502 using Tapatalk